New mechanism of membrane fusion

We have carried out Monte Carlo simulation of the fusion of bilayers of single chain amphiphiles which show phase behavior similar to that of biological lipids. The fusion mechanism we observe is very different from the ``stalk'' hypothesis. Stalks do form on the first stage of fusion, but they do not grow radially to form a hemifused state. Instead, stalk formation destabilizes the membranes and results in hole formation in the vicinity of the stalks. When holes in each bilayer nucleate spontaneously next to the same stalk, an incomplete fusion pore is formed. The fusion process is completed by propagation of the initial connection, the stalk, along the edges of the aligned holes.Comment: 4 pages, 3 figure

Fluctuations in mixtures of lamellar- and nonlamellar-forming lipids

We consider the role of nonlamellar-forming lipids in biological membranes by examining fluctuations, within the random phase approximation, of a model mixture of two lipids, one of which forms lamellar phases while the other forms inverted hexagonal phases. To determine the extent to which nonlamellar-forming lipids facilitiate the formation of nonlamellar structures in lipid mixtures, we examine the fluctuation modes and various correlation functions in the lamellar phase of the mixture. To highlight the role fluctuations can play, we focus on the lamellar phase near its limit of stability. Our results indicate that in the initial stages of the transition, undulations appear in the lamellae occupied by the tails, and that the nonlamellar-forming lipid dominates these undulations. The lamellae occupied by the head groups pinch off to make the tubes of the hexagonal phase. Examination of different correlations and susceptibilities makes quantitative the dominant role of the nonlamellar-forming lipids.Comment: 7 figures (better but larger in byte figures are available upon resuest), submitte

Phase Separation of Saturated and Mono-unsaturated Lipids as determined from a Microscopic Model

A molecular model is proposed of a bilayer consisting of fully saturated DPPC and mono unsaturated DOPC. The model not only encompasses the constant density within the hydrophobic core of the bilayer, but also the tendency of chain segments to align. It is solved within self-consistent field theory. A model bilayer of DPPC undergoes a main chain transition to a gel phase, while a bilayer of DOPC does not do so above zero degrees centigrade because of the double bond which disrupts order. We examine structural and thermodynamic properties of these membranes and find our results in reasonable accord with experiment. In particular, order-parameter profiles are in good agreement with NMR experiments. A phase diagram is obtained for mixtures of these lipids in a membrane at zero tension. The system undergoes phase separation below the main-chain transition temperature of the saturated lipid. Extensions to the ternary DPPC, DOPC, and cholesterol system are outlined.Comment: 29 pages, 4 figures, 1 table. revised versio

Membrane Heterogeneity: Manifestation of a Curvature-Induced Microemulsion

To explain the appearance of heterogeneities in the plasma membrane, I propose a hypothesis which begins with the observation that fluctuations in the membrane curvature are coupled to the difference between compositions in one leaf and the other. Because of this coupling, the most easily excited fluctuations can occur at non-zero wavenumbers. When the coupling is sufficiently strong, it is well-known that it leads to microphase separation and modulated phases. I note that when the coupling is less strong, the tendency towards modulation remains manifest in a liquid phase that exhibits transient structure of a characteristic size; that is, it is a microemulsion. The characteristic size of the fluctuating domains is estimated to be on the order of 100 nm, and experiments to verify this hypothesis are proposed.Comment: 4 page

The strong Novikov conjecture for low degree cohomology

We show that for each discrete group G, the rational assembly map K_*(BG) \otimes Q \to K_*(C*_{max} G) \otimes \Q is injective on classes dual to the subring generated by cohomology classes of degree at most 2 (identifying rational K-homology and homology via the Chern character). Our result implies homotopy invariance of higher signatures associated to these cohomology classes. This consequence was first established by Connes-Gromov-Moscovici and Mathai. Our approach is based on the construction of flat twisting bundles out of sequences of almost flat bundles as first described in our previous work. In contrast to the argument of Mathai, our approach is independent of (and indeed gives a new proof of) the result of Hilsum-Skandalis on the homotopy invariance of the index of the signature operator twisted with bundles of small curvature.Comment: 11 page